New Zealand Fungarium - Te Kohinga Hekaheka o Aotearoa

PDD 80311

Packet

Active

20 December 2004

24 February 2023

Haematonectria illudens

D.P. Mahoney

Neocosmospora illudens (Berk.) L. Lombard & Crous

Ascomycota

Sordariomycetes

Hypocreales

Nectriaceae

Determination

has host Coprosma sp.

bark of dead branch

Coprosma sp.

Coprosma

Tracheophyta

Magnoliopsida

Gentianales

Rubiaceae

Determination

no

Unknown

Lower Hutt, 45 Gurney Road, bush area

Latitude and Longitude (WGS84):  -41.2187  174.935 

Lower Hutt, 45 Gurney Road, Bush area

D.P. Mahoney

Daniel P. Mahoney

AEB861

2004/01/01

2004-01-01

Wellington

New Zealand

Ngāti Toa Rangatira

Rangitāne

Taranaki Whānui ki te Upoko o te Ika

Te Atiawa (Wellington)

New Zealand Map Grid:  2672255E 5996870N  (WGS84 -41.218719 174.935374)

Oedocephalum glomerulosum hyperparasite also present. Conidiophores numerous, separate or clustered, simple, robust, smooth, hyaline, with occasional thin septa but always with a prominent septum just below the terminal vesicle, slightly tapering to the point below the vesicle, 300-500 µm long X 11-12 µm wide below the vesicle. Vesicle ellipsoid-obovoid 41-50 X 38-41 µm (n=2), conidia produced synchronously over the vesicle surface from short triangular pegs (which remain as scars about 1.5 µm above the vesicle surface after conidia fall away). Conidia large, smooth, hyaline, one-celled, broadly obovoid with very rounded apices, 16-23 X 11-16 µm (n=11). Substrate: Dead branch (ca. 4-5 inches in diameter) of a Coprosma tree species with the reddish to reddish orange perithecia numerous of the intact bark. Collection date: First observed in January, 2004, but then the log was placed under the steps leading to the garage and forgotten until early March. The H. illudens was still in excellent shape due to the wet weather in February. Voucher material: herbarium specimen (the bark was easily removed with a razor blade), several SMF semipermanent slides and numerous colored projection slides -- habit views (in situ on the bark) and higher magnification views of asci, ascospores, etc. Ann\lquotes herbarium number is AB861 =PDD 80311. Brief description: Perithecia numerous and gregarious over the surface of the dead bark and among the moss, superficial, , globose to subglobose and fairly uniform in appearance, ca. 450 µm in diameter; red, especially when younger and before the more yellowish to yellowish-orange warty patches predominated on the peridial surface \endash sometimes reddish, even at the later very warty stage, when perithecia were moist (some older perithecia were yellowish orange even when moist); most perithecia dull yellowish to yellowish orange as they dried. Perithecia not collapsing or becoming cupulate as they dried. Peridium yellowish in lactic acid mounts and red in Melzer\lquotes mounts, becoming randomly covered by first a few and then many yellowish patches of large globular to irregularly angular, thick-walled cells (the walls yellowish), 22-28 µm in diam with walls 2-3 µm thick, peridial cells below these patches globular to angular but smaller and not as thick walled, reddish, becoming less pigmented, flattened and more angular in the inner peridium. Ostiole surrounded by an unusual palisade of narrow, vertically elongated, thick-walled, colorless cells which persisted as a unit even after the perithecium had been squashed (sometimes this \rquoteostiolar unit\lquote could be seen as a clear very slightly domed area at the top of the perithecium, and sometimes not \endash but no warts formed in this area so the position of the ostiole on the perithecium was readily apparent). Periphyses were easily seen lining the inner edge of this ostiole-forming area. Asci cylindrically clavate, 8- and 4-spored (the latter predominating in some perithecia and always present even when 8-spored asci predominated), spores arranged irregularly biseriately in the upper portion of the asci and uniseriate in the lower portion (4-spored asci were always uniseriate), apices with a non-amyloid plug through which there seemed to be a \rquoteclearer\lquote canal (no clear \rquotering\lquote was evident), no ascus measurements were made. Paraphyses not characterized. Ascospores ellipsoid to broadly fusiform, 2-celled with a median transverse septum (usually not invaginated at the septum, or only slightly so, although a number of \rquoteabberant\lquote spores were deeply indented at the septum, on one side only, and there was a tendency for these and for non- or slightly indented spores to disarticulate when pressure was exerted on the coverslip), apices rounded, sometimes slightly tapered, hyaline at first but dull orangish at maturily, ornamented with delicate, but distinct, longitudinal striations and containing 1-few large guttules and many tiny guttules/cell, 24-30 X 11-13. Addendum: During the final selection of herbarium material and preparation of SMF voucher slides (12 March 2004), the following observations were made. 1) By far the majority of ascospores seen today, and previously for this species, have been smaller than the mature ascospores, more delicately striate and unpigmented. Fully mature ascospores were never seen in their asci but were often seen loose within older perithecia \endash sometimes in abundance, but usually not. Perhaps full maturity isn\lquotet reached until the ascospores are discharged? Only mature, pigmented, striate ascospores were measured. Considering the many slides I made from a wide variety of perithecia in this large collection, I was surprised that there were so few fully mature ascospores. 2) Ascospore germination was observed. At first I saw only disarticulated spores germinating from their truncated ends. These were of nearly mature size but still hyaline. Later I mounted a dull yellowish orange perithecium which contained a number of intact (not disarticulated) fully mature germinating spores \endash among the abundant mature ascus-free ascospores within. The germ tubes (one/ascospore) were emerging from the side of one cell only \endash not in the region of the septum. 3) A few Fusarium macroconidia were observed in one mount. Although this nectrioid species has a Fusarium anamorph, I have no way of knowing whether these represented that anamorph. One spore was measured at 50 µm long and was 6-celled \endash with 5 evenly spaced transverse septa. 4) With perithecial decline, by natural aging or through mite or insect attack, only a deep cup with a warty crust remained \endash the hole at the top of this cup being sometimes small and sometimes much larger.