ICMP 13301 – Pantoea agglomerans (Ewing & Fife 1972) Gavini et al.
Data provider:
International Collection Of Microorganisms
Barcode:
ICMP 13301
Culture type:
Bacterial Culture
Database record added:
25 January 2002
Database record updated:
04 September 2024
Components
Primary component
Active identification
Determined name:
Erwinia herbicola (Löhnis 1911) Dye 1964
Determiner:
Identification date:
Preferred name:
Pantoea agglomerans (Ewing & Fife 1972) Gavini et al.
Division:
Pseudomonadota
Class:
Gammaproteobacteria
Order:
Enterobacterales
Family:
Erwiniaceae
Identification type:
Determination
Associations:
isolated from Malus X domestica Borkh.
Other identifications
Identification
Determined name:
Erwinia herbicola
Determiner:
Identification date:
Preferred name:
Pantoea agglomerans (Ewing & Fife 1972) Gavini et al.
Active:
no
Identification type:
Received as
Other components
Active identification
Determined name:
Malus X domestica Borkh.
Determiner:
Identification date:
Preferred name:
Malus domestica
Division:
Tracheophyta
Class:
Magnoliopsida
Order:
Rosales
Family:
Rosaceae
Vernacular name:
apple cv. Golden Delicious
Identification type:
Determination
Part affected:
blossom
Present:
no
Collection events
Primary collection event
Collection event type:
Field
Standard locality
Location:
Loburn
Georeferences:
Latitude and Longitude (WGS84):
-43.244 172.553
Verbatim locality:
Loburn
Verbatim collector:
L. Kearns
Standardised collector:
Leah Kearns
Collectors reference no.:
Eh1087
Verbatim date:
Nov 1989
Start date:
1989-11
Country:
New Zealand
New Zealand Area Codes:
North Canterbury
Native lands:
Ngāi Tahu
Georeferences:
Latitude and Longitude (WGS84):
-43.244 172.553
Culture flags
Price Category:
Category 1
Culture notes
Other Details:
inhibitory to Erwinia amylovora in immature pear fruit
Avirulent to:
Non-pathogenic
Note:
A naturally-occurring Erwinia herbicola strain, Eh1087, inhibitory to Erwinia amylovora (Burr.) Winslow et al. (1920) was isolated from apple blossom (Malus X. domestica Borkh.) in a Canterbury orchard. Total populations of E. herbicola on apple blossoms in Canterbury and Hawkes Bay orchards were low (less than 50 cfu.blossom-1) throughout flowering, increasing l00-fold at petal drop. E. Herbicola populations at petal drop were more dominant in Canterbury orchards (30-50% of total) than in Hawkes Bay orchards (1-2% of total) in the seasons surveyed. In vitro inhibition of E. amylovora was observed for 20% of all bacterial strains isolated from Canterbury orchards and from none of the strains isolated from Hawkes Bay orchards under the conditions used. Eh1087 established stable populations for at least 10 days post application when sprayed onto apple blossoms in the orchard. This strain produced a broad spectrum, non-peptide, β-lactam antibiotic in vitro that was bactericidal for E. amylovora. Eh1087 suppressed E. amylovora disease symptoms in immature pear fruit, excised apple shoots and in apple blossoms. The partially purified antibiotic of Eh1087 reduced the severity of symptom expression in immature pear fruit. TnphoA-insertion mutants of Eh1087 were created (Ant-) that failed to inhibit E. amylovora in vitro and in immature pear fruit. Ant- mutants no longer produced an antibiotic inhibitory to E. amylovora in vitro. TnphoA insertions in the Ant- mutants all mapped within a 1.5 kb region on a 200 kb indigenous plasmid of Ehl087. A genomic library of Eh1087 was constructed and cosmids which complemented Ant- mutants to the Ant+ phenotype in vitro and in immature pear fruit were selected. A HinIII 8 kb cosmid fragment subclone, pAH8, weakly complemented Ant- mutant EhA17g. This complementation was enhanced by the presence in trans of an EcoR1 5 kb cosmid fragment subclone, pBE5B, which mapped approximately 6 kb away from the region of TnphoA insertions. Analysis of the proteins produced by these cosmid fragment sub clones in Escherichia coli mini-cells revealed that pBE5B codes for three proteins, approximately 20, 34 and 41 kd in size and pAH8 codes for two proteins, approximately 28 and 39 kd in size. At least one of the proteins coded for by pAH8 must be essential to antibiotic synthesis in Eh1087. The possible relationship between the cosmid fragment-encoded proteins and b-lactam biosynthetic enzymes is discussed.
Leah Kearns, 1993
Leah Kearns, 1993
External links
Culture events
Isolated:
Actioned by:
Leah Kearns (Reference number Eh1087)
Date:
01 November 1989 (Verbatim: 01 Nov 1989)
Deposited:
Note:
Derived from earliest batch date
Actioned by:
Leah Kearns (Reference number 1087)
Date:
01 July 1997 (Verbatim: 01 Jul 1997)
Permissions
Project permits
Biocultural (BC) Notice
